Coding
Part:BBa_K4724007:Design
Designed by: Qingqing Zheng Group: iGEM23_NJTech-China-A (2023-10-06)
IsPETaseT116R-6xHis Tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 790
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal AgeI site found at 546 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We needed to purify the IsPETase protein, so we attached the 6xHis tag after IsPETase.
Source
It's a new part, which is a mutant of IsPETase from Ideonella sakaiensis.